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cyanine7 amine cy7  (Lumiprobe)


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    Structured Review

    Lumiprobe cyanine7 amine cy7
    Cyanine7 Amine Cy7, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy7+amine/Cyanine7+amine/pm42754783-55-0-8
    Average 94 stars, based on 37 article reviews
    cyanine7 amine cy7 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: Molecular Jackhammers Eradicate Cancer Cells by Vibronic-Driven Action
    Article Snippet: Cy7.5-amine, Cy7-amine, Cy5.5-amine and Cy5-amine were purchased from Lumiprobe Corp. (Maryland, USA).

    Fluorescence:

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment.
    Article Snippet: .. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment
    Article Snippet: .. Nanoparticle solutions (100 μg/mL) prepared in SGF (pH 3.6) were then deposited onto the mucin-coated silicon wafers and incubated for 15 min. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Labeling:

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment.
    Article Snippet: .. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment
    Article Snippet: .. Nanoparticle solutions (100 μg/mL) prepared in SGF (pH 3.6) were then deposited onto the mucin-coated silicon wafers and incubated for 15 min. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Conjugation Assay:

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment.
    Article Snippet: .. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment
    Article Snippet: .. Nanoparticle solutions (100 μg/mL) prepared in SGF (pH 3.6) were then deposited onto the mucin-coated silicon wafers and incubated for 15 min. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Article Title: Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming
    Article Snippet: C57BL/6 mice (Jackson Laboratory, female, 8 weeks, Cat #000664) were i.v. injected 100 μL of PBAE/mRNA particles formulated with Cy5-labeled mRNA (ApexBio Technology, Cat. #R1010) and Cy7-labeled plasmid DNA (Cy7-pDNA) at a weight ratio of 4:1, and with a dose of 0.25 mg mRNA/kg. .. The Cy7- pDNA was synthesized following a previous report8 utilizing photo-reactive conjugation of SPB (Sigma, Cat. #803545) onto pDNA and subsequent reaction with Cy7-amine (Lumiprobe, Cat. #150C0). ..

    Article Title: Cold Atmospheric Plasma-Activated In Situ Hydrogel Induces Hair Regeneration Via Immune Microenvironment Remodeling.
    Article Snippet: .. The solution was adjusted to pH 4.7 and stirred for 3 h. Subsequently, 0.525 μmol of Cy3-amine or Cy7-amine (Lumiprobe Corp, Hunt Valley, MD, USA) were introduced under continuous stirring, and the conjugation reaction proceeded overnight. .. To prepare CAPgel/IL2, 0.1 mL of HTsol (10 mg mL−1) containing IL2 (3.0 μg mL−1) was irradiated with CAP for 150 s. The IL2 content in CAPgel/IL2 was quantified using an IL2 enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA).

    Incubation:

    Article Title: Self-disassembling nanoparticles as oral nanotherapeutics targeting intestinal microenvironment
    Article Snippet: .. Nanoparticle solutions (100 μg/mL) prepared in SGF (pH 3.6) were then deposited onto the mucin-coated silicon wafers and incubated for 15 min. For fluorescence observation, melanin nanoparticles were fluorescently labeled with Cy7-amine (Lumiprobe, cat# 250C0) through EDC click chemistry likewise Cy5 conjugation. ..

    Synthesized:

    Article Title: Supramolecular assembly of polycation/mRNA nanoparticles and in vivo monocyte programming
    Article Snippet: C57BL/6 mice (Jackson Laboratory, female, 8 weeks, Cat #000664) were i.v. injected 100 μL of PBAE/mRNA particles formulated with Cy5-labeled mRNA (ApexBio Technology, Cat. #R1010) and Cy7-labeled plasmid DNA (Cy7-pDNA) at a weight ratio of 4:1, and with a dose of 0.25 mg mRNA/kg. .. The Cy7- pDNA was synthesized following a previous report8 utilizing photo-reactive conjugation of SPB (Sigma, Cat. #803545) onto pDNA and subsequent reaction with Cy7-amine (Lumiprobe, Cat. #150C0). ..



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    a Schematic illustration of the in vivo biocompatibility imaging of sHAChiF with various formulations by luminol assay. b Representative IVIS images of bright field (left) and bioluminescence (right) of luminol activity for implanted sHAChiF in C57BL/6 mice (Scale bar = 10 mm). c Quantification of bioluminescence efficiencies of luminol activities for implanted sHAChiF in the mice. d Representative IVIS images of fluorescence by Cy5.5-conjugated Chi (Chi Cy5.5 ; cyan; pseudocolor), and <t>Cy7.5-conjugated</t> sHA (sHA Cy7.5 ; magenta; pseudocolor) in sHAChiF at various time points (Scale bar = 10 mm). e In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in sHAChiF over time through calculation of fluorescence intensity, normalized by fluorescence at 0 d. f Quantification of normalized sHAChiF in vivo retention at 7 d and 14 d. Data are shown as mean ± SD (n = 3) independent samples in b , c ; n = 4 independent samples in d – f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of c , f . Source data are provided as a Source Data file. Image was created using PowerPoint.
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    Fig. 3. In-situ freezing microscopy of [mPEG-b-p(HPMAm-Bz)]-based polymeric micelles with and without cryoprotectants. A) Fluorescence microscopy images of dry ice-induced in-situ freezing of <t>Cy7-labelled</t> mPEG-b-p(HPMAm-Bz) micelles before, during freezing (frozen) and after thawing (thawed), in the absence (without) and presence of cryoprotectants (sucrose or trehalose). B) Cryogenic-scanning electron microscopy (cryo-SEM) of frozen fractured mPEG-b-p(HPMAm-Bz) micelles (green arrows) in the presence of sucrose (orange arrow) or trehalose (purple arrow) compared to non-cryoprotected micelles (without). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    a Schematic illustration of the in vivo biocompatibility imaging of sHAChiF with various formulations by luminol assay. b Representative IVIS images of bright field (left) and bioluminescence (right) of luminol activity for implanted sHAChiF in C57BL/6 mice (Scale bar = 10 mm). c Quantification of bioluminescence efficiencies of luminol activities for implanted sHAChiF in the mice. d Representative IVIS images of fluorescence by Cy5.5-conjugated Chi (Chi Cy5.5 ; cyan; pseudocolor), and Cy7.5-conjugated sHA (sHA Cy7.5 ; magenta; pseudocolor) in sHAChiF at various time points (Scale bar = 10 mm). e In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in sHAChiF over time through calculation of fluorescence intensity, normalized by fluorescence at 0 d. f Quantification of normalized sHAChiF in vivo retention at 7 d and 14 d. Data are shown as mean ± SD (n = 3) independent samples in b , c ; n = 4 independent samples in d – f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of c , f . Source data are provided as a Source Data file. Image was created using PowerPoint.

    Journal: Nature Communications

    Article Title: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications

    doi: 10.1038/s41467-024-52753-0

    Figure Lengend Snippet: a Schematic illustration of the in vivo biocompatibility imaging of sHAChiF with various formulations by luminol assay. b Representative IVIS images of bright field (left) and bioluminescence (right) of luminol activity for implanted sHAChiF in C57BL/6 mice (Scale bar = 10 mm). c Quantification of bioluminescence efficiencies of luminol activities for implanted sHAChiF in the mice. d Representative IVIS images of fluorescence by Cy5.5-conjugated Chi (Chi Cy5.5 ; cyan; pseudocolor), and Cy7.5-conjugated sHA (sHA Cy7.5 ; magenta; pseudocolor) in sHAChiF at various time points (Scale bar = 10 mm). e In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in sHAChiF over time through calculation of fluorescence intensity, normalized by fluorescence at 0 d. f Quantification of normalized sHAChiF in vivo retention at 7 d and 14 d. Data are shown as mean ± SD (n = 3) independent samples in b , c ; n = 4 independent samples in d – f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of c , f . Source data are provided as a Source Data file. Image was created using PowerPoint.

    Article Snippet: Cyanine5.5 (Cy5.5) NHS ester (Catalog # 27020) and Cyanine7 (Cy7) amine (Catalog # 260C0) were purchased from Lumiprobe (USA).

    Techniques: In Vivo, Imaging, Activity Assay, Fluorescence

    a Representative images of the aggregation behavior of rat peritoneal macrophages following treatment with various polysaccharides (Scale bar = 50 μm). b Quantification of aggregated rat peritoneal macrophages treated with various polysaccharides. c Schematic illustration of the mouse hepatic thermal injury model. d Representative images of mouse organs in the mouse hepatic thermal injury model after treatment with sHAChiF, showing F4/80 (yellow; pseudocolor), Chi (cyan; pseudocolor), and sHA (magenta; pseudocolor) (Scale bar = 10 mm). e Quantification of F4/80 expression on the wound by measuring fluorescence with APC/Fire TM anti-F4/80 antibody, normalized by fluorescence levels in the normal liver. f Quantification fluorescence of sHA from sHA Cy7.5 on the wound (left) and Chi from Chi Cy5.5 on the wound (right), normalized by fluorescence from normal liver. g Schematic illustration of analysis for sHA uptake behavior of peritoneal cells using flow cytometry. Peritoneal cells gated based on sHA + population ( h ), grouping of sHA + LPM and sHA + SPM by F4/80 and CD11b expression levels ( i ), the ratio of peritoneal cell populations distinguishing between sHA - and sHA + populations ( j ). Data are shown as mean ± SD ( n = 3) independent samples in a , b ; n = 4 independent samples in d – f ; n = 4 independent samples in h – j . Two-tailed unpaired t-test was used for statistical analysis of f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of b , e . Source data are provided as a Source Data file. Image was created using PowerPoint.

    Journal: Nature Communications

    Article Title: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications

    doi: 10.1038/s41467-024-52753-0

    Figure Lengend Snippet: a Representative images of the aggregation behavior of rat peritoneal macrophages following treatment with various polysaccharides (Scale bar = 50 μm). b Quantification of aggregated rat peritoneal macrophages treated with various polysaccharides. c Schematic illustration of the mouse hepatic thermal injury model. d Representative images of mouse organs in the mouse hepatic thermal injury model after treatment with sHAChiF, showing F4/80 (yellow; pseudocolor), Chi (cyan; pseudocolor), and sHA (magenta; pseudocolor) (Scale bar = 10 mm). e Quantification of F4/80 expression on the wound by measuring fluorescence with APC/Fire TM anti-F4/80 antibody, normalized by fluorescence levels in the normal liver. f Quantification fluorescence of sHA from sHA Cy7.5 on the wound (left) and Chi from Chi Cy5.5 on the wound (right), normalized by fluorescence from normal liver. g Schematic illustration of analysis for sHA uptake behavior of peritoneal cells using flow cytometry. Peritoneal cells gated based on sHA + population ( h ), grouping of sHA + LPM and sHA + SPM by F4/80 and CD11b expression levels ( i ), the ratio of peritoneal cell populations distinguishing between sHA - and sHA + populations ( j ). Data are shown as mean ± SD ( n = 3) independent samples in a , b ; n = 4 independent samples in d – f ; n = 4 independent samples in h – j . Two-tailed unpaired t-test was used for statistical analysis of f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of b , e . Source data are provided as a Source Data file. Image was created using PowerPoint.

    Article Snippet: Cyanine5.5 (Cy5.5) NHS ester (Catalog # 27020) and Cyanine7 (Cy7) amine (Catalog # 260C0) were purchased from Lumiprobe (USA).

    Techniques: Expressing, Fluorescence, Flow Cytometry, Two Tailed Test

    a Schematic illustrations of modeling rat ischemic button model with sHAChiF (top) and representative images of sHAChiF treated on the ischemic buttons (Scale bar = 10 mm). b Representative images of rat ischemic button model on d 0 and d 7 after surgery (Scale bar = 10 mm). c Representative Masson’s trichrome staining images of adhesive tissues on d 7 after surgery (Scale bar = 1 mm). d Specification of scoring severity of peritoneal adhesion. e Adhesion formation rates with adhesion scores of sHAChiF per rat. f Quantification of the adhesion scores of dual-sprayable hydrogels. g Representative IVIS images of fluorescence by Chi Cy5.5 (cyan; pseudocolor) and sHA Cy7.5 (magenta; pseudocolor) in dual-sprayable hydrogels at various time points (Scale bar = 50 mm). h In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in dual-sprayable hydrogels over time through calculation of fluorescence intensity, normalized by fluorescence at d 0. i Representative iNOS and Arg1 immunofluorescence staining images of adhesive tissues on d 7 after surgery (Scale bar = 50 μm). j Quantification of M1/M2 ratio of peritoneal macrophages in adhesive tissues on d 7 after surgery. Quantification of pro-inflammatory cytokines IL−1β ( k ), IL-6 ( l ), and TNF-α ( m ) from rat serum on d 4 after surgery. Data are shown as mean ± SD ( n = 5 independent samples in b – f ; n = 4 independent samples in g , h ; n = 5 independent samples in i , j ; n = 4 independent samples in k – m ). One-way ANOVA with Tukey post-hoc test was used for statistical analysis of e , f , j – m . Source data are provided as a Source Data file. Image was created using PowerPoint.

    Journal: Nature Communications

    Article Title: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications

    doi: 10.1038/s41467-024-52753-0

    Figure Lengend Snippet: a Schematic illustrations of modeling rat ischemic button model with sHAChiF (top) and representative images of sHAChiF treated on the ischemic buttons (Scale bar = 10 mm). b Representative images of rat ischemic button model on d 0 and d 7 after surgery (Scale bar = 10 mm). c Representative Masson’s trichrome staining images of adhesive tissues on d 7 after surgery (Scale bar = 1 mm). d Specification of scoring severity of peritoneal adhesion. e Adhesion formation rates with adhesion scores of sHAChiF per rat. f Quantification of the adhesion scores of dual-sprayable hydrogels. g Representative IVIS images of fluorescence by Chi Cy5.5 (cyan; pseudocolor) and sHA Cy7.5 (magenta; pseudocolor) in dual-sprayable hydrogels at various time points (Scale bar = 50 mm). h In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in dual-sprayable hydrogels over time through calculation of fluorescence intensity, normalized by fluorescence at d 0. i Representative iNOS and Arg1 immunofluorescence staining images of adhesive tissues on d 7 after surgery (Scale bar = 50 μm). j Quantification of M1/M2 ratio of peritoneal macrophages in adhesive tissues on d 7 after surgery. Quantification of pro-inflammatory cytokines IL−1β ( k ), IL-6 ( l ), and TNF-α ( m ) from rat serum on d 4 after surgery. Data are shown as mean ± SD ( n = 5 independent samples in b – f ; n = 4 independent samples in g , h ; n = 5 independent samples in i , j ; n = 4 independent samples in k – m ). One-way ANOVA with Tukey post-hoc test was used for statistical analysis of e , f , j – m . Source data are provided as a Source Data file. Image was created using PowerPoint.

    Article Snippet: Cyanine5.5 (Cy5.5) NHS ester (Catalog # 27020) and Cyanine7 (Cy7) amine (Catalog # 260C0) were purchased from Lumiprobe (USA).

    Techniques: Staining, Adhesive, Fluorescence, In Vivo, Immunofluorescence

    Fig. 3. In-situ freezing microscopy of [mPEG-b-p(HPMAm-Bz)]-based polymeric micelles with and without cryoprotectants. A) Fluorescence microscopy images of dry ice-induced in-situ freezing of Cy7-labelled mPEG-b-p(HPMAm-Bz) micelles before, during freezing (frozen) and after thawing (thawed), in the absence (without) and presence of cryoprotectants (sucrose or trehalose). B) Cryogenic-scanning electron microscopy (cryo-SEM) of frozen fractured mPEG-b-p(HPMAm-Bz) micelles (green arrows) in the presence of sucrose (orange arrow) or trehalose (purple arrow) compared to non-cryoprotected micelles (without). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Microfluidic formulation, cryoprotection and long-term stability of paclitaxel-loaded π electron-stabilized polymeric micelles.

    doi: 10.1016/j.jconrel.2024.08.041

    Figure Lengend Snippet: Fig. 3. In-situ freezing microscopy of [mPEG-b-p(HPMAm-Bz)]-based polymeric micelles with and without cryoprotectants. A) Fluorescence microscopy images of dry ice-induced in-situ freezing of Cy7-labelled mPEG-b-p(HPMAm-Bz) micelles before, during freezing (frozen) and after thawing (thawed), in the absence (without) and presence of cryoprotectants (sucrose or trehalose). B) Cryogenic-scanning electron microscopy (cryo-SEM) of frozen fractured mPEG-b-p(HPMAm-Bz) micelles (green arrows) in the presence of sucrose (orange arrow) or trehalose (purple arrow) compared to non-cryoprotected micelles (without). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Cyanine 7-amine (Cy7) dye was ordered from Lumiprobe (Germany).

    Techniques: In Situ, Microscopy, Fluorescence, Electron Microscopy